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rabbit polyclonal anti-serping1 antibody (GeneTex)

Name: rabbit polyclonal anti-serping1 antibody
Brand: GeneTex
Rating: 90 (1 reviews)

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GeneTex rabbit polyclonal anti-serping1 antibody
Details of the primers used for quantitative real-time RT-PCR analysis

Rabbit Polyclonal Anti Serping1 Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti-serping1 antibody/product/GeneTex
Average 90 stars, based on 1 article reviews

rabbit polyclonal anti-serping1 antibody - by Bioz Stars, 2026-02

90/100 stars

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1) Product Images from "Differential gene expression of serine protease inhibitors in bovine ovarian follicle: possible involvement in follicular growth and atresia"

Article Title: Differential gene expression of serine protease inhibitors in bovine ovarian follicle: possible involvement in follicular growth and atresia

Journal: Reproductive Biology and Endocrinology : RB&E

doi: 10.1186/1477-7827-9-72

Figure Legend Snippet: Details of the primers used for quantitative real-time RT-PCR analysis

Techniques Used: Quantitative RT-PCR

QPCR analysis of SERPINA5, SERPINB6, SERPINE1, SERPINE2, SERPINF2 and SERPING1 in healthy and atretic follicles . Total RNA from the follicular wall (i.e., granulosa plus theca interna) was extracted from three healthy follicles and three atretic follicles. The expression of mRNA was normalized to the expression of GAPDH measured in the same RNA preparation. The black and white bars indicate healthy and atretic follicles, respectively. Data are shown as the mean ± SEM. Different superscript letters denote significant differences ( P < 0.05).

Figure Legend Snippet: QPCR analysis of SERPINA5, SERPINB6, SERPINE1, SERPINE2, SERPINF2 and SERPING1 in healthy and atretic follicles . Total RNA from the follicular wall (i.e., granulosa plus theca interna) was extracted from three healthy follicles and three atretic follicles. The expression of mRNA was normalized to the expression of GAPDH measured in the same RNA preparation. The black and white bars indicate healthy and atretic follicles, respectively. Data are shown as the mean ± SEM. Different superscript letters denote significant differences ( P < 0.05).

Techniques Used: Expressing

mRNA localization of SERPINA5, SERPINB6, SERPINF2 and SERPING1 in E 2 -active and E 2 -inactive follicles . SERPINA5, SERPINB6 and SERPINF2 mRNA was expressed more in healthy than in atretic follicles, while SERPING1 mRNA was expressed more in atretic than in healthy follicles in QPCR analysis. (A, C, E, G, I, K, M and O) Digoxigenin (DIG)-labeled anti-sense cRNA probes were used. (B, D, F, H, J, L, N and P) DIG-labeled sense cRNA probes were used. Sections (7 μm) of bovine follicles were hybridized with each probe. SERPINA5 (A, B, C and D), SERPINB6 (E, F, G and H) and SERPINF2 (I, J, K and L) mRNA were found in the GCs of E 2 -active follicles and a weak hybridization signal was detected in GCs of E 2 -inactive follicles. SERPING1 mRNA (M, N, O and P) was detected in both GCs and the TL of E 2 -inactive follicles and a weak hybridization signal was also detected in both GCs and the TL of E 2 -active follicles. Scale bars = 20 μm.

Figure Legend Snippet: mRNA localization of SERPINA5, SERPINB6, SERPINF2 and SERPING1 in E 2 -active and E 2 -inactive follicles . SERPINA5, SERPINB6 and SERPINF2 mRNA was expressed more in healthy than in atretic follicles, while SERPING1 mRNA was expressed more in atretic than in healthy follicles in QPCR analysis. (A, C, E, G, I, K, M and O) Digoxigenin (DIG)-labeled anti-sense cRNA probes were used. (B, D, F, H, J, L, N and P) DIG-labeled sense cRNA probes were used. Sections (7 μm) of bovine follicles were hybridized with each probe. SERPINA5 (A, B, C and D), SERPINB6 (E, F, G and H) and SERPINF2 (I, J, K and L) mRNA were found in the GCs of E 2 -active follicles and a weak hybridization signal was detected in GCs of E 2 -inactive follicles. SERPING1 mRNA (M, N, O and P) was detected in both GCs and the TL of E 2 -inactive follicles and a weak hybridization signal was also detected in both GCs and the TL of E 2 -active follicles. Scale bars = 20 μm.

Techniques Used: Labeling, Hybridization

Protein localization of SERPINA5, SERPINB6, SERPINF2 and SERPING1 in E 2 -active and E 2 -inactive follicles . Localization of SERPINA5 (A and B), SERPINB6 (C and D), SERPINF2 (E and F) and SERPING1 (G and H) protein was detected by immunohistochemistry. Sections (7 μm) of bovine E 2 -active (A, C, E and G) and E 2 -inactive follicles (B, D, F and H) were incubated with anti-SERPINA5, anti-SERPINB6, anti-SERPINF2 and anti-SERPING1 polyclonal antibodies. SERPINA5, SERPINB6 and SERPINF2 were detected in the GCs of E 2 -active and E 2 -inactive follicles. SERPING1 was detected in both GCs and the TL of E 2 -active and E 2 -inactive follicles. Negative control (I and J) was incubated without anti-SERPIN antibodies. Scale bars = 20 μm.

Figure Legend Snippet: Protein localization of SERPINA5, SERPINB6, SERPINF2 and SERPING1 in E 2 -active and E 2 -inactive follicles . Localization of SERPINA5 (A and B), SERPINB6 (C and D), SERPINF2 (E and F) and SERPING1 (G and H) protein was detected by immunohistochemistry. Sections (7 μm) of bovine E 2 -active (A, C, E and G) and E 2 -inactive follicles (B, D, F and H) were incubated with anti-SERPINA5, anti-SERPINB6, anti-SERPINF2 and anti-SERPING1 polyclonal antibodies. SERPINA5, SERPINB6 and SERPINF2 were detected in the GCs of E 2 -active and E 2 -inactive follicles. SERPING1 was detected in both GCs and the TL of E 2 -active and E 2 -inactive follicles. Negative control (I and J) was incubated without anti-SERPIN antibodies. Scale bars = 20 μm.

Techniques Used: Immunohistochemistry, Incubation, Negative Control

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Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Differential gene expression of serine protease inhibitors in bovine ovarian follicle: possible involvement in follicular growth and atresia

doi: 10.1186/1477-7827-9-72

Figure Lengend Snippet: Details of the primers used for quantitative real-time RT-PCR analysis

Article Snippet: The 7-μm-thick follicular sections were incubated at room temperature for 4 h with rabbit polyclonal anti-SERPINA5 antibody (H00005104, Abnova, Taipei, Taiwan) diluted 1:10, rabbit polyclonal anti-SERPINB6 antibody (GTX114637, GeneTex Inc, Irvine, CA, USA) diluted 1:100, rabbit polyclonal anti-SERPINF2 antibody (H00005345, Abnova) diluted 1:20 or rabbit polyclonal anti-SERPING1 antibody (GTX105316, GeneTex) diluted 1:300 in Discovery Ab diluents (Roche).

Techniques: Quantitative RT-PCR

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Differential gene expression of serine protease inhibitors in bovine ovarian follicle: possible involvement in follicular growth and atresia

doi: 10.1186/1477-7827-9-72

Figure Lengend Snippet: QPCR analysis of SERPINA5, SERPINB6, SERPINE1, SERPINE2, SERPINF2 and SERPING1 in healthy and atretic follicles . Total RNA from the follicular wall (i.e., granulosa plus theca interna) was extracted from three healthy follicles and three atretic follicles. The expression of mRNA was normalized to the expression of GAPDH measured in the same RNA preparation. The black and white bars indicate healthy and atretic follicles, respectively. Data are shown as the mean ± SEM. Different superscript letters denote significant differences ( P < 0.05).

Techniques: Expressing

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Differential gene expression of serine protease inhibitors in bovine ovarian follicle: possible involvement in follicular growth and atresia

doi: 10.1186/1477-7827-9-72

Figure Lengend Snippet: mRNA localization of SERPINA5, SERPINB6, SERPINF2 and SERPING1 in E 2 -active and E 2 -inactive follicles . SERPINA5, SERPINB6 and SERPINF2 mRNA was expressed more in healthy than in atretic follicles, while SERPING1 mRNA was expressed more in atretic than in healthy follicles in QPCR analysis. (A, C, E, G, I, K, M and O) Digoxigenin (DIG)-labeled anti-sense cRNA probes were used. (B, D, F, H, J, L, N and P) DIG-labeled sense cRNA probes were used. Sections (7 μm) of bovine follicles were hybridized with each probe. SERPINA5 (A, B, C and D), SERPINB6 (E, F, G and H) and SERPINF2 (I, J, K and L) mRNA were found in the GCs of E 2 -active follicles and a weak hybridization signal was detected in GCs of E 2 -inactive follicles. SERPING1 mRNA (M, N, O and P) was detected in both GCs and the TL of E 2 -inactive follicles and a weak hybridization signal was also detected in both GCs and the TL of E 2 -active follicles. Scale bars = 20 μm.

Techniques: Labeling, Hybridization

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Differential gene expression of serine protease inhibitors in bovine ovarian follicle: possible involvement in follicular growth and atresia

doi: 10.1186/1477-7827-9-72

Figure Lengend Snippet: Protein localization of SERPINA5, SERPINB6, SERPINF2 and SERPING1 in E 2 -active and E 2 -inactive follicles . Localization of SERPINA5 (A and B), SERPINB6 (C and D), SERPINF2 (E and F) and SERPING1 (G and H) protein was detected by immunohistochemistry. Sections (7 μm) of bovine E 2 -active (A, C, E and G) and E 2 -inactive follicles (B, D, F and H) were incubated with anti-SERPINA5, anti-SERPINB6, anti-SERPINF2 and anti-SERPING1 polyclonal antibodies. SERPINA5, SERPINB6 and SERPINF2 were detected in the GCs of E 2 -active and E 2 -inactive follicles. SERPING1 was detected in both GCs and the TL of E 2 -active and E 2 -inactive follicles. Negative control (I and J) was incubated without anti-SERPIN antibodies. Scale bars = 20 μm.

Techniques: Immunohistochemistry, Incubation, Negative Control

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1) Product Images from "Differential gene expression of serine protease inhibitors in bovine ovarian follicle: possible involvement in follicular growth and atresia"

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